AviTri™

Three colors DNA loading dye

AviTri™:
Elevate Your Gel Electrophoresis with Three-Color Precision

Gel electrophoresis is a cornerstone technique in molecular biology, enabling researchers to separate and analyze DNA fragments based on size.
To streamline this process and enhance visualization, scientists rely on DNA loading dyes. These specialized solutions are added to DNA samples before they’re loaded onto agarose or polyacrylamide gels.

Understanding DNA Loading Dyes:
More Than Just Density

DNA loading dyes play a multifaceted role in gel electrophoresis, offering several critical advantages beyond simply increasing sample density:

1. Density Adjustment: Loading dyes contain high-density substances (e.g., glycerol, ficoll) that ensure DNA samples settle neatly at the bottom of the well, preventing them from floating away during electrophoresis.

2. Visual Tracking: The colored dyes within the loading dye co-migrate with DNA fragments during electrophoresis. This allows researchers to monitor the progress of the run in real time and estimate the approximate size of DNA fragments.

3. pH Indication: Some loading dyes also contain pH indicators, verifying that the sample’s pH is compatible with the electrophoresis buffer, ensuring optimal conditions for separation.

4. Nucleic Acid Protection: Certain loading dyes may include components like EDTA, which chelate divalent cations and help protect DNA samples from degradation by nucleases.

The Three-Color Advantage: Unlocking Precision and Clarity

While single and dual-color loading dyes are commonly used, three-color dyes like AviTri™ revolutionize the electrophoresis experience:

Dual-Color vs. Three-Color:

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Why Choose Three Colors?

• Enhanced Resolution: The third dye in AviTri™, Orange G, migrates exceptionally fast, allowing for the precise tracking and resolution of even the smallest DNA fragments (10-20 base pairs). This is crucial in applications like microsatellite analysis or analysis of short PCR products.
• Optimized Run Time: The three distinct migration rates of the dyes (Orange G, Bromophenol Blue, and Xylene Cyanol) provide multiple reference points, enabling researchers to accurately judge when to stop the electrophoresis run, preventing smaller fragments from running off the gel.
• Increased Versatility: AviTri™ is ideal for a wider range of applications, including those involving the analysis of complex DNA mixtures. The three colors can be used to differentiate between different sample types loaded on the same gel.

AviTri™:
A New Benchmark in DNA Loading Dyes

AviTri™ combines the best features of traditional loading dyes with the enhanced precision and versatility of a three-color system. Its carefully optimized formulation boasts numerous benefits:

• Superior Accuracy: The three distinct colors provide unparalleled accuracy in tracking DNA migration and estimating fragment sizes.
• Exceptional Clarity: The dyes produce sharp, well-defined bands on agarose gels, simplifying the interpretation of results.
• Ready-to-Use Convenience: AviTri™ is pre-mixed and ready to use, saving valuable time in the lab.
• Broad Compatibility: Compatible with various gel electrophoresis systems and buffers.
• Minimized Diffusion: The dyes in AviTri™ are formulated to minimize diffusion, ensuring sharp bands even after extended electrophoresis runs.

Unleash the Full Potential of Your Gel Electrophoresis with AviTri™

AviTri™ empowers researchers to achieve a higher level of precision and efficiency in their gel electrophoresis workflows. Its unique three-color system, optimized for resolving even the smallest DNA fragments, sets a new standard for accuracy and convenience in DNA analysis.

Experience the difference AviTri™ can make in your lab.
Contact us today for more information or to request a sample.

• 
  Orange G Dye runs faster than Bromophenol blue or xylene
  cyanol FF dyes in standard agarose gels.
• 
  Orange G dye migrates with DNA between 10 and 20 nucleotides long.

Molecular Biology Kits

Easy cDNA Synthesis Kit
2X SYBR® Green Real Time PCR
2X Taq PCR Master Mix
One-Step SYBR RT-QPCR Mix

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How to use

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