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Hot Start Taq DNA Polymerase

AviAHot™

avigene

Keep in freezer (≥ -20°C)

Apta Hot-Start Taq DNA Polymerase

AviAHot ™ , Apta Hot Start Taq DNA Polymerase is a mixture of Taq DNA polymerase and a temperature sensitive aptamer-based inhibitor.
The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40 °C, but releases the enzyme during normal PCR cycling conditions.
The aptamer-based hot start mechanism does not require a separate high temperature incubation step to activate the enzyme.
AviAHot™ is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.
The activated AviAHot™ maintains the same functionality as Taq DNA polymerase:
It catalyzes 5′ → 3′ synthesis of DNA, has no detectable 3′ → 5′ proofreading exonuclease activity.

$58.00 – $169.00

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SKU: A10510-A10511-A10512

Related documents of AviHot™

AviHot™– ManualDownload
Avigene PCR & RT-PCR Enzymes & Reagents– BrochureDownload

Apta-Hot-Start-Taq-DNA-Polymerase1
Apta-Hot-Start-Taq-DNA-Polymerase

AviHot™

AviHot™ is a mixture of Taq DNA polymerase and a temperature sensitive, aptamer -based inhibitor.
The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40 °C, but releases the enzyme during normal PCR cycling conditions.

 


  • Reduction of primer dimers.

  • No inactivation time.

  • Avoid non-specific bands.

  • More stable than antibody based Hot-start taqs.

  • More economical than antibody based Hot-start taqs.

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