AviAHot™
Keep in freezer (≥ -20°C)
Hot Start Taq DNA Polymerase – Apta
AviAHot ™ , Apta Hot Start Taq DNA Polymerase is a mixture of Taq DNA polymerase and a temperature sensitive aptamer-based inhibitor.
The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40 °C, but releases the enzyme during normal PCR cycling conditions.
The aptamer-based hot start mechanism does not require a separate high temperature incubation step to activate the enzyme.
AviAHot™ (Apta Hot Start Taq DNA Polymerase) is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.
The activated AviAHot™ maintains the same functionality as Taq DNA polymerase:
It catalyzes 5′ → 3′ synthesis of DNA, has no detectable 3′ → 5′ proofreading exonuclease activity.
Original price was: $58.00.$29.90Current price is: $29.90.
Related documents of AviAHot™
| AviAHot™– Manual | Download |
|---|---|
| Avigene PCR & RT-PCR Enzymes & Reagents– Brochure | Download |
AviAHot™ (Hot Start Taq DNA Polymerase – Apta)
AviAHot™ is a mixture of Taq DNA polymerase and a temperature sensitive, aptamer -based inhibitor.
The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40 °C, but releases the enzyme during normal PCR cycling conditions.
Reduction of primer dimers.
No inactivation time.
Avoid non-specific bands.
More stable than antibody based Hot-start taqs.
More economical than antibody based Hot-start taqs.
Frequently asked questions
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A seemingly elegant design can quickly begin to bloat with unexpected content or break under the weight of actual activity. Fake data can ensure a nice looking layout but it doesn’t reflect what a living, breathing application must endure. Real data does.
Websites in professional use templating systems. Commercial publishing platforms and content management systems ensure that you can show different text, different data using the same template. When it’s about controlling hundreds of articles, product pages for web shops.
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