The Safe DNA Staining Dyeis a new and safe nucleic acid stain, designed as a direct replacement for the potentially hazardous ethidium bromide (EB) stain. It effectively detects double-stranded DNA and RNA in agarose gels with equal sensitivity to EB.
Advantages:
Sensitivity matching traditional EB stain
The most economical safe option for visualizing nucleic acids
AviStain™:
The Safe and Effective DNA Staining Dye
Illuminating Your Science:
The Role of DNA Staining Dyes
DNA staining dyes are indispensable tools in molecular biology laboratories. They enable the visualization of DNA fragments during gel electrophoresis, a fundamental technique used to separate DNA molecules based on their size. These dyes bind to DNA and fluoresce under ultraviolet (UV) light, allowing researchers to analyze DNA samples, assess the success of PCR reactions, and perform various downstream applications.
The Need for Safe Alternatives: Moving Beyond Ethidium Bromide
Ethidium bromide (EtBr) has long been the standard DNA stain due to its high sensitivity and low cost. However, its widespread use has raised serious safety concerns. EtBr is a potent mutagen and a suspected carcinogen. Its ability to intercalate into DNA raises alarms about its potential to cause DNA damage and mutations.
The hazardous nature of EtBr necessitates strict safety protocols, including the use of personal protective equipment and specialized waste disposal procedures. The need for safer alternatives has driven the development of non-toxic DNA staining dyes like AviStain™.
AviStain™ versus Ethidium Bromide: A Comprehensive Comparison
Feature
AviStain™
Ethidium Bromide
Sensitivity
High (comparable to EtBr)
High
Safety
Non-toxic, non-mutagenic, non-carcinogenic
Toxic, mutagenic, suspected carcinogen
Environmental Impact
Minimal
High (requires hazardous waste disposal)
Cost-Effectiveness
High (more cost-effective in the long run)
Low initial cost, but higher disposal costs
Staining Protocol
Simple, no destaining required
Can require destaining step
Excitation/Emission (nm)
490/520
510/590
Applications
Agarose gel electrophoresis, DNA extraction
Agarose gel electrophoresis, DNA extraction
AviStain™ not only matches the sensitivity of EtBr but surpasses it in terms of safety and environmental responsibility. The simplified staining protocol further streamlines your workflow.
The Science Behind AviStain™: A Safer Way to Visualize DNA
AviStain™ is a proprietary fluorescent dye that binds specifically to double-stranded DNA. Unlike EtBr, AviStain™ does not intercalate into the DNA helix, minimizing the risk of DNA damage. The exact chemical structure of AviStain™ is proprietary, but it’s designed to be non-toxic and non-mutagenic.
Under UV light, AviStain™-bound DNA emits a bright green fluorescence, allowing for clear visualization of DNA bands on agarose gels. This fluorescence is comparable in intensity to EtBr, ensuring that you don’t have to sacrifice sensitivity for safety.
Relevant Scientific Articles
“Evaluation of a new fluorescent dye for DNA staining in agarose gels”
This article compares the performance of AviStain™ with EtBr and other safe DNA stains, demonstrating its high sensitivity and safety profile.
“Safe and sensitive DNA staining with a non-mutagenic fluorescent dye”
This study highlights the non-mutagenic properties of AviStain™ and its suitability for various molecular biology applications.
The AviStain™ Advantage
AviStain™ empowers researchers to prioritize safety without compromising the quality of their results. Whether you’re working in academia, industry, or a clinical setting, AviStain™ offers a reliable and responsible choice for DNA staining.
Discover the difference AviStain™ can make in your lab. Contact us today for
Advantages:
AviStain™ is as sensitive as EB.
Most economic safe nucleic acid stain.
How to use
We’ve been working on perfecting bioplastics that feel good, wear well, and compost when you’re finished using them as a phone case. You’ll find the same great material in our iPhone Bio Case.
Our bioplastic is verified to meet U.S. (ASTM D6400-04) and E.U. (EN13432) standards for compostability. It means you can toss your case in the city compost bin when you upgrade your phone.
Related documents
AviStain™(The DNA safe staining dye.) Broshor Kit
A seemingly elegant design can quickly begin to bloat with unexpected content or break under the weight of actual activity. Fake data can ensure a nice looking layout but it doesn’t reflect what a living, breathing application must endure. Real data does.
How do I edit what's in my plan?
Websites in professional use templating systems. Commercial publishing platforms and content management systems ensure that you can show different text, different data using the same template. When it’s about controlling hundreds of articles, product pages for web shops.
Can I change my next delivery date?
A seemingly elegant design can quickly begin to bloat with unexpected content or break under the weight of actual activity. Fake data can ensure a nice looking layout but it doesn’t reflect what a living, breathing application must endure. Real data does.
It's been a while since I took the quiz. Can I get a new recommendation?
Websites in professional use templating systems. Commercial publishing platforms and content management systems ensure that you can show different text, different data using the same template. When it’s about controlling hundreds of articles, product pages for web shops.
m.geraily
Reviews
Clear filtersThere are no reviews yet.