AviRex™ Blood RNA

Keep in the refrigerator (+4°C ~ +8°C)

Blood RNA Extraction Kit 

AviRex™ Blood RNA is designed for the silica spin-based isolation of total intracellular RNA from up to 200 μl of fresh or frozen whole blood treated with common anticoagulants like heparin, EDTA, or acid-citrate-dextrose. The streamlined procedure effectively removes contaminants and enzyme inhibitors, ensuring fast, convenient, and reliable isolation of total RNA.

Cell lysis, RNase inactivation, and DNA removal are accomplished using a phenol-based solution. Following the separation of the RNA-containing fraction and the addition of an RNA enhancer, the lysate is applied to a spin column. This step effectively washes away cellular debris and contaminants such as hemoglobin, leaving behind high-quality RNA, which is then eluted in DEPC-treated water.

Advantages:

    • Minimal Sample Size: Requires only 200 μl of blood
    • Streamlined Protocol: Fast and easy procedure
    • DNA Depletion: Effectively removes DNA contamination
    • Optimal Yield: Typical yield of 1-4 µg of RNA

$169.00

SKU: N/A

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AviRex™
Blood RNA Extraction Kit.

Uncover the Dynamic Language of Life with AviRex™ Blood RNA Extraction Kit

Blood RNA (ribonucleic acid) is the messenger of our genes, actively translating the genetic code within our DNA into the proteins that build and maintain our bodies. Unlike the stable DNA blueprint, RNA is dynamic, fluctuating in response to our environment, health, and even the time of day. By extracting and analyzing blood RNA, we gain a real-time snapshot of our biological processes and unlock a deeper understanding of health, disease, and the intricate workings of our cells.

AviRex™ Blood RNA:
Your Key to Gene Expression Insights

AviRex™ Blood RNA revolutionizes how we access this vital molecular snapshot. Our kit provides a simple and efficient method for isolating high-quality RNA from blood samples. Whether you’re a researcher studying gene expression patterns, a healthcare professional seeking biomarkers for disease diagnosis, or an individual curious about the inner workings of your own body, AviRex™ Blood RNA empowers you to explore the dynamic world of RNA.

Blood RNA versus Other RNA Sources: A Unique Perspective

While RNA is present in all living cells, blood RNA offers a unique window into the human body’s overall physiological state. Here’s what sets it apart from RNA extracted from other sources like plants or tissues:

  • Accessibility and Relevance: Blood is readily accessible through routine medical procedures, making it a convenient and non-invasive source of RNA. The RNA present in blood reflects the activity of various tissues and organs, providing a comprehensive overview of the body’s gene expression landscape.

  • Dynamic Nature: Blood RNA levels can change rapidly in response to various factors, including disease, medication, stress, and environmental influences. This dynamic nature makes blood RNA an invaluable tool for monitoring health, diagnosing diseases, and tracking responses to treatment.

  • Diagnostic Potential: Blood RNA can serve as a source of biomarkers – specific RNA molecules whose presence or absence can indicate the presence of a particular disease or condition. This opens up new avenues for early disease detection and personalized medicine.

Preserving the Integrity of Blood RNA: Essential Steps

RNA is notoriously delicate and prone to degradation. To ensure the quality and reliability of your RNA extraction with AviRex™ Blood RNA, it’s crucial to follow these essential steps:

  • Prompt Sample Processing: Process your blood sample as quickly as possible after collection to minimize RNA degradation. If immediate processing is not feasible, store the sample at -80°C.

  • RNase Inactivation: Use RNase inhibitors during the extraction process to prevent the breakdown of RNA by enzymes called RNases.

  • Cold Storage: Store extracted RNA at -80°C in a dedicated RNA freezer to maintain its stability and prevent degradation.

  • Avoid Freeze-Thaw Cycles: Repeated freezing and thawing can damage RNA molecules. Aliquot your RNA into smaller volumes to minimize the need for multiple freeze-thaw cycles.

  • Work in a Clean Environment: Use sterile techniques and dedicated RNA-free equipment to minimize the risk of RNase contamination.

The Power of Blood RNA Extraction: A Multifaceted Tool

The applications of blood RNA extraction are vast and transformative:

  • Disease Diagnostics and Prognostics: Identify RNA biomarkers for early disease detection, monitor disease progression, and predict treatment response.

  • Pharmacogenomics: Understand how individuals respond to medications based on their genetic makeup, leading to more personalized and effective drug therapies.

  • Infectious Disease Research: Detect and monitor viral infections, track the emergence of new viral strains, and develop antiviral therapies.

  • Cancer Research: Identify RNA signatures associated with different types of cancer, develop targeted cancer therapies, and monitor treatment efficacy.

  • Immunology: Study the immune system’s response to infections, vaccines, and autoimmune diseases.

The AviRex™ Blood RNA Advantage:
Your Trusted Partner in RNA Research

AviRex™ Blood RNA empowers researchers and healthcare professionals with a reliable and efficient tool for blood RNA extraction. Our kit is designed to deliver:

  • High-Quality RNA: Isolate pure, intact RNA suitable for a wide range of downstream applications, including RT-PCR, RNA sequencing, and microarray analysis.
  • Ease of Use: Follow our streamlined protocol for consistent and reproducible results, even for those with limited experience in RNA extraction.
  • Expert Support: Our team of scientists and technical specialists is available to answer your questions and provide guidance throughout the process.

  • Low sample size 200 μl

  • Fast and easy protocol

  • DNA depletion

  • Suitable yield 1-4 μg

AviRex™ Blood RNA
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Blood RNA Extraction Kit.

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